【摘要】 目的探讨缺血预处理(IPC)对减体积肝移植大鼠再灌注损伤早期细胞凋亡的影响。方法建立50%减体积大鼠肝移植模型,将72只成年雄性SD大鼠随机分为两组:对照组和IPC组,检测术后2、6、24 h血清丙氨酸转氨酶(ALT)水平变化及组织病理学变化;TUNEL法检测术后24 h肝细胞凋亡指数;免疫组织化学检测bcl-2和caspase-3蛋白表达。结果与对照组比较,IPC组术后6、24 h ALT水平下降,术后24 h肝损伤减轻,肝细胞凋亡指数、caspase-3表达均下降,而抗凋亡蛋白bcl-2表达增加(P均<0.01)。结论 IPC明显减轻减体积肝移植术后再灌注损伤,其机制与通过上调bcl-2、下调caspase-3蛋白表达,从而抑制肝脏细胞凋亡相关。更多还原

【Abstract】 Objective To investigate the effect of ischemic preconditioning(IPC) on early hepatocellular apoptosis after reperfusion injury in reduced-size liver transplantation model and its significance.Methods We performed 50% sized rat liver transplantation model.A total of 72 adult male SD rats were randomly divided into two groups: control group and IPC group.Serum ALT levels at 2nd,6th and 24th hour were examined and histopathological changes were analyzed.Apoptotic index of hepatocytes in liver grafts at 24 th hour after reperfusion were examined by using TUNEL assay.The expression of bcl-2 and caspase-3 protein were examined by method of immunohistochemistry.Results Compared with control group,we observed significantly changes in IPC group,including serum ALT levels decreased at 6th and 24th hour after reperfusion,minimal liver damaged,apoptotic index of hepatocytes and caspase-3 expression reduced,as well as the expression of antiapoptotic pretein bcl-2 increased.Conclusion IPC could ameliorate early ischemia reperfusion injury in reduced-size liver grafts.It’s protective mechanisms might be mediated in part by prohibiting the early hepatocellular apoptosis,which due to the upregulate of bcl-2 and inhibite of caspase-3.更多还原