【摘要】 目的观察鼻咽癌(NPC)组织中EB病毒潜伏膜蛋白1(LMP1)和hsa-miR-19b表达变化及其与NPC临床病理特征的关系。方法采用免疫组化技术检测46例NPC放疗前活检标本中LMP1的表达情况,用实时定量PCR检测hsa-miR-19b表达情况。分析二者表达相关性及其与NPC临床病理特征的关系。结果NPC组织中LMP1阳性表达率为60.95%,hsa-miR-19b阳性表达量为68.27±69.00;LMP1、hsa-miR-19b表达均与NPC分期和淋巴结转移有关。LMP1表达与hsa-miR-19b表达呈正相关(r=0.390,P=0.007)。结论LMP1可能调控hsa-miR-19b的表达,两者的表达呈正相关关系。LMP1、hsa-miR-19b的高表达与NPC分期和转移有关。更多还原

【Abstract】 Objective To study the relationship between the expression of LMP1 and hsa-miR-19b;and the relationship between their expression and clinicopathological parameters.Methods LMP1 of 46 cases of nasopharyngeal carcinoma was detected by immunohistochemisty and hsa-miR-19b by RT-PCR.Results The positive expression rate of LMP1 was 60.95%(28/46).The relative expression value of hsa-miR-19b was 68.27±69.00.Both LMP1 and hsa-miR-19b were associated with stage and lymphatic metastasis.The expression of LMP1 were positively associated with the expression of hsa-miR-19b(r=0.390,P=0.007).Conclusions LMP1 may play a regulation role in the expression of hsa-miR-19b and their expression are possitively correlation.High expression of LMP1 and hsa-miR-19b may have prognosis significance in staging and metastasis in nasopharyngeal carcinoma.更多还原