【摘要】 目的通过研究KAI1基因在人类喉鳞状细胞癌及转移淋巴结中的蛋白表达情况,试图阐明KAI1基因蛋白的表达与喉癌转移之间的关系。方法取喉鳞状细胞癌组织标本蜡块40例及转移的淋巴结13例,以癌旁正常组织标本蜡块30例作为对照,采用免疫组织化学SP法,检测KAI1单克隆抗体在喉鳞状细胞癌、转移淋巴结及癌旁正常组织标本中蛋白的表达,并结合患者临床病理资料进行统计学分析。结果KAI1基因在癌旁正常组织、喉鳞状细胞癌及转移淋巴结中的表达率分别为93.33%(28/30)、62.50%(25/40)、30.77%(4/13),且各组之间差异均有统计学意义(P<0.05)。KAI1基因的蛋白表达与喉鳞状细胞癌的临床分期差异有统计学意义(P<0.05),与性别、年龄、原发部位、病理学分级及有无淋巴结转移无关(P>0.05)。结论KAI1基因在喉鳞状细胞癌转移淋巴结中的表达较在喉鳞状细胞癌中的表达呈显著性下降,该基因可能在喉癌转移过程中起一定作用。更多还原

【Abstract】 Objective To find the association between expression of the KAI1 gene and metastasis of laryngeal carcinoma by determining expression of the KAI1 protein in human laryngeal squamous cell carcinomas and metastatic lymph nodes.Methods Using the immunohistochemical S-P technique,we studied the expression of KAI1 in 40 laryngeal squamous cell carcinoma specimens,13 metastatic lymph node specimens and 30 carcinoma normal side tissue specimens with the monoclonal antibody of KAI1.Correlations of expression of the KAI1 gene to clinicopathological factors were statistically analyzed.Results The KAI1-positive rates in carcinoma normal side tissues,laryngeal squamous cell carcinoma and metastatic lymph nodes were 93.33%(28/30),62.5%(25/40)and 30.77%(4/13)respectively,and the differences were statistically significant(P<0.05).Expression of the KAI1 gene was related to the clinical stage(P<0.05).There was no relationship of expression of the KAI1 gene to the patients’ gender,the patients’ age,the primary site of tumor,pathological grades and lymphatic metastasis(P>0.05).Conclusion There is a significant reduction in expression of the KAI1 gene in metastatic lymph nodes compared with laryngeal squamous cell carcinomas.The KAI1 gene may play a role in the metastasis of laryngeal carcinomas.更多还原