【摘要】 目的检测肝细胞生长因子(HGF)和血管内皮生长因子C(VEGF-C)在非小细胞肺癌(NSCLC)组织中的表达,分析两者的相关性及其与NSCLC淋巴管生成的关系。方法应用免疫组织化学SP法,检测72例NSCLC患者及20例癌旁正常肺组织中HGF和VEGF-C的表达,用D2-40标记淋巴管,计数微淋巴管密度(LMVD),分析HGF和VEGF-C共表达情况及与LMVD和临床病理参数之间的关系。结果 HGF和VEGF-C在NSCLC中癌组织的表达明显高于癌旁正常肺组织(31.94%vs15.00%、48.65%vs25.00%,P均<0.05),且两者存在明显正相关(r=0.170,P<0.05);HGF或VEGF-C阳性表达的LMVD显著增高[HGF:(16.98±4.31)vs(13.06±4.28),P<0.05;VEGF-C:(19.43±3.24)vs(14.66±3.51),P<0.05],且与患者淋巴结转移成正相关(P均<0.05)。结论 HGF和VEGF-C在NSCLC中存在共表达,且两者均与肿瘤淋巴管的生成及淋巴道的转移密切相关。更多还原

【Abstract】 Objective To determine expressions of hepatocyte growth factor(HGF) and vascular endothelial growth factor-c(VEGF-C) and their relevance,and evaluate the relationship with lymphangiogenesis in non-small cell lung cancer(NSCLC).Methods Applying the immunohistochemical SP method,lymphatic vessels and expressions of HGF and VEGF-C were detected in 72 non-small cell lung cancer tissues and 20 adjacent normal tissues.Lymphatic micro-vessel density(LMVD) was counted with D2-40 as the specific marker of the lymphatic endothelium.The relationship of HGF and VEGF-C with LMVD and the clinicopathological features of NSCLC were then analyzed.Results HGF/VEGF-C positive expression rates were significantly higher than those in adjacent normal tissues(31.94% vs 15.00%,48.65% vs 25.00%,both P<0.05),and there was a positive correlation between expressions of HGF and VEGF-C(r=0.170,P<0.05).Higher LMVD was found in NSCLC with HGF or VEGF-C positive expression [HGF:(16.98±4.31) vs(13.06±4.28),P<0.05;VEGF-C:(19.43±3.24) vs(14.66±3.51),P<0.05].Expressionsof HGF and VEGF-C were positively correlated with lymph node metastasis(both P<0.05).Conclusion In non-small cell lung cancer,co-expression of HGF and VEGF-C can promote lymphangiogenesis and lymphatic metastasis.更多还原