【摘要】 目的探讨TRB3基因+251A/G多态性与代谢综合征(MS)患者左房功能改变的相关性。方法选择MS患者177例,正常对照组156例。采用酚/氯仿法提取全血DNA,PCR-RFLP法检验受试者TRB3基因第二外显子+251A/G的多态性位点。应用应变率成像技术测量左房各壁的应变率。结果 +251A/G位点AA、AG+GG基因型和等位基因的频率分布在正常对照组和MS组,两组间差异有统计学意义(P均<0.05);与正常对照组相比,MS组左房平均收缩期应变率峰值(mean SSR)、平均舒张早期应变率峰值(mean ESR)显著降低(P均<0.001),提示MS患者左房储存器和管道功能显著减低,左房平均舒张晚期应变率峰值(mean ASR)差异无统计学意义(P>0.05),MS患者左房助力泵功能无显著变化;与AA基因型相比,AG+GG基因型者左房mean SSR、mean ESR和mean ASR均明显降低(P均<0.05);多元线性回归分析显示mean ESR、mean ASR与TRB3基因+251A/G多态性G等位基因之间存在数量依存关系(P<0.05)。结论 TRB3基因+251A/G多态性G等位基因与MS患者左房功能受损相关,在MS患者左房功能损害中可能起重要作用。更多还原

【Abstract】 Objective To investigate the association of the TRB3 gene +251A /G polymorphism with alterations of left atrial function in patietns with metabolic syndrome(MS).Methods A total of 177 MS patients and 156 gender-and age-matched healthy subjects as controls were included.Genomic DNA was obtained from peripheral blood leukocytes using standard phenol-chloroform extraction procedures.The TRB3 gene + 251A /G polymorphism was determined for all participants by the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method.Strain rate imaging was preformed to assess left atrial function.Results The distributions of genotypic and allelic frequencies of the TRB3 gene + 251A /G polymorphism showed significant differences between the MS group and controls(all P <0.05).Compared with controls,the decreases in mean SSR and ESR,reflecting impairment of the LA reservoir and conduit function in patients with MS,were significant(both P < 0.001).However,mean ASR,an indicator of LA booster function,was similar in the two groups(P > 0.05).This finding suggested there were no changes in the boost-er function of the left atrium.The carriers of the G allele(AG and GG genotypes) demonstrated significantly lower mean SSR,mean ESR and mean ASR of the left atrium than those in carriers of the AA genotype in the MS group(both P <0.05).Multiple linear regression analysis showed that the G allele of the TRB3 gene + 251A/G polymor-phism significantly correlated with mean ESR and mean ASR of LA(both P < 0.05).Conclusion The TBB3 gene + 251A /G polymorphism has been associated with alterations of LA function.The G allele of the + 251A /G polymor-phism could be an important risk factor for deteriorations of LA function in the patients with metabolic syndrome.更多还原