【摘要】 目的探讨联合检测诱导痰和纤维支气管镜活组织端粒酶活性对肺癌的诊断价值。方法采用端粒重复序列扩增-酶联免疫吸附实验方法 ,对80例肺癌患者和50例肺部良性病变患者,分别检测其诱导痰和纤维支气管镜活组织端粒酶的活性。结果肺癌患者诱导痰和纤维支气管镜活组织端粒酶的活性均明显高于肺部良性病变患者(P<0.001),不同病理类型的肺癌患者两种标本的端粒酶活性差异无统计学意义(P>0.05)。诱导痰和纤维支气管镜活组织端粒酶活性的检测对肺癌的诊断敏感性、特异性、准确性分别为62.5%(50/80)和60.0%(48/80)、72.0%(36/50)和70.0%(35/50)、66.2%(86/130)和63.8%(83/130);两项联合检测的敏感性、特异性和准确性分别为82.5%(66/80)、64.0%(32/50)和75.4%(98/130)。结论诱导痰和纤维支气管镜活组织端粒酶活性的联合检测具有更高的敏感性,可以提高肺癌的诊断率。更多还原

【Abstract】 Objective To investigate the significance of determination of telomerase activity in induced sputum and fiberobronchoscopic biopsy samples in lung cancer patients. Methods The technique of TRAP(telomeric repeat amplification protocal)-PCR-ELISA was employed to detect telomerase levels in induced sputum and fiberobronchoscopic biopsy samples in 80 lung cancer patients and 50 benign lung disease patients. Results Telomerase levels in induced sputum and fiberobronchoscopic biopsy samples were higher in patients with lung cancer than in those with benign lung disease(P<0.001).There was no significant difference in telomerase activity between the different pathological types(P>0.05).The sensitivity,specificity and overall accuracy of telomerase activity was 62.5% (50/80),72.0 % (36/50) and 66.2%(86/130) in induced sputum,and 60.0%(48/80),70.0%(35/50),and 63.8%(83/130) in fiberobronchoscopic biopsy samples respectively. The sensitivity,specificity and overall accuracy of telomerase activity simultaneously determined by the above methods was 82.5%(66/80),64.0%(32/50)and 75.4%(98/130) respectively. Conclusion Determination of telomerase activity in induced sputum and fiberobronchoscopic biopsy samples has high sensitivity and can improve the diagnostic accuracy for lung cancer.更多还原