【摘要】 以野生(♂)和人工养殖(♀)黄颡鱼杂交的100个F1个体为作图群体,用SSR、SRAP和TRAP3种DNA分子标记技术构建黄颡鱼的遗传连锁图谱。图谱整合了13个SSR标记,89个SRAP标记,26个TRAP标记。其中雌性框架图谱包括16个连锁群,图谱的长度为585.5cM;雄性框架图谱包括15个连锁群,图谱的长度为752.3cM;共享框架图谱包括5个连锁群,图谱的长度为231.3cM。用该连锁图谱对黄颡鱼的5个生长相关性状进行QTL扫描,在雌性图谱上检测到1个头宽的QTL,定位于第七连锁群上,LOD值为3.2,可解释的表型变异为13%。在雄性图谱上分别检测到1个体高和体长的QTL,均定位于第一连锁群上。体高QTL的LOD值为2.4,可解释的表型变异为12%。全长QTL的LOD值为2.1,可解释的表型变异为11%。3个QTL均可用于黄颡鱼的生长性状的标记辅助育种。更多还原

【Abstract】 Genetic linkage maps of yellow catfish (Pelteobagrus fulvidraco) were constructed using SSR,SRAP and TRAP markers and a pseudo-testcross mapping strategy. A segregating population including 100 progenies was sampled within the first filial generation obtained by artificial fertilization between a male parent and a female parent from wild and breeding populations respectively. Totally,13 SSR,89 SRAP and 26 TRAP markers were used to construct a genetic map. Female map included 16 linkage groups,covering a total of 585.5 cM in length. Male map included 15 linkage groups,covering a total of 752.3 cM in length. Combined map included 5 linkage groups,covering a total of 231.3 cM in length. This map was used to scan the QTL related to growth traits of yellow catfish. One head length QTLs were identified in female map located in LG7,LOD value is 3.2 and accounted for 13 % of phenotypic variation. A body width and a body length QTLs were identified in male map both located in LG1,LOD value is 2.4 and 2.1 respectively,and accounted for 12% and 11% of phenotypic variation respectively. Three QTLs could be considered to apply in marker-assisted selection for growth-related traits breeding of yellow catfish.更多还原