【摘要】 将黑腹果蝇(Drosophila melanogaster)抗真菌肽基因Drosomycin(Drs)克隆到pPICZα-A载体中,构建分泌型表达载体pPICZα-A-Drs,转化宿主菌Pichia pastoris X-33.在AOX1(醇氧化酶)启动子调控下,抗真菌肽DRS成功表达,其分子量约为5 kD.抑菌试验显示,DRS对供试真菌有明显的抑菌活性.采用考马斯亮蓝法测定抗真菌肽的具体表达量,并优化了诱导条件.更多还原

【Abstract】 The Drosophila melanogaster antifungal peptide gene Drosomycin(Drs),was expressed in Pichia pastoris X-33 transformed by the recombinant expression vector pPICZα-A-Drs,which was combined by vector pPICZα-A and Drs.This resulting vector pPICZα-A-Drs contained a-Factor under the control of the alcohol oxidase 1 promoter.Following induction with methanol,the DRS peptide was synthesized with a molecular weight of 5.0 kD in a soluble form.Antifungal assay showed that the DRS had a obvious inhibitive effect on tested fungi.The amount of expression product DRS were determined by the coomassie blue staining,and the conditions of the expression were optimized.更多还原