【摘要】 目的为研究在脊髓急性损伤中,自组装短肽溶液对创伤后神经组织的保护作用及创伤后早期神经组织细胞凋亡的时间分布规律。方法实验使用了手术切断大鼠脊髓胸段T8至T10作为脊髓损伤动物模型,使用末端脱氧核苷酸转移酶介导生物素标记(TUNEL)技术,检测实验后不同时间点的损伤脊髓组织细胞凋亡情况。结果伤后2h,损伤区及邻近段开始出现末端标记阳性细胞;伤后8h,凋亡阳性细胞数达高峰;同时,自组装短肽实验组细胞凋亡较空白对照组少。结论自组装短肽溶液对急性脊髓创伤组织具有保护作用。更多还原

【Abstract】 Objective To explore self-assembly peptides characteristics to apoptosis following spinal cord injury. Methods Rat model of spinal cord injury was established. Apoptosis in the injured area was examined by using terminal deoxynucleotidyl-mediated nick-end labeling(TUNEL). Results The TUNEL positive cells were detected 2 hours post injury,the number of TUNEL-positive stain reached a maximum 8 hours after injury,and there were fewer TUNEL-positive cells detected in self-assembling peptide group(SAP group). Conclusions Self-assembly peptides do benefit to depress cell apoptosis in the spinal cord after injury.更多还原