【摘要】 在单因素试验的基础上,根据Box-Behnken的中心组合实验设计原理,采用3因素3水平的响应曲面分析法,建立了胰蛋白酶水解酪蛋白制备酪蛋白磷酸肽的二次多项数学模型,并以水解度为响应值作响应面和等高线,得到酶水解酪蛋白制备酪蛋白磷酸肽的优化工艺条件为:水解时间为6 h,酶底比为2‰,温度50℃,底物质量分数为4%,pH值为8.4,旋转振荡速度120 r/min,在此条件下实际水解度为62.84%。更多还原

【Abstract】 By single factor experiment,the proper hydrolysis time、temperature、substrate concentration and enzyme-substrate ratio were deter-mined.The quadratic function mathematics model,for the processing of CPP from trypsin hydrolyzed casein,was bult,according to the Box-Behnken design principles,by three factors of exercise at three levels response surface methodology,with the Minitab14 statistics analysis software.By analyzing the response surface and contour based on the degree of hysrolysis as response value,the optimal processing was obtained as follows: hydrolysis time 6 h、substrate concentration and enzyme-substrate ratio 2‰,temperature 50 degree celsius、substrate concentration 4%,pH 8.4,with 120 r/min,and the actual degree of hysrolysis 62.84%.更多还原