【摘要】 调查云南泸西县汉族群体17个Y-STR基因座的多态性分布,探讨其群体遗传学及法医学应用价值。应用AmpFSTRYfiler荧光标记复合扩增系统扩增156名汉族无关男性个体的17个Y-STR基因座,用ABI3100遗传分析仪进行基因检测,计算等位基因及单倍型频率,并结合已公开发表的国内外其他16个群体的遗传学资料,分析各群体间的遗传距离。云南泸西汉族男性群体中,共观察到154种单倍型,其中152种为仅观察到1次的单倍型,2种单倍型观察到2次,单倍型多样性(HD)值为0.9998,基因多样性(GD)值在0.3901(DYS437)—0.9632(DYS385a/b)。17个群体遗传距离分析提示,国内人群云南泸西汉族与湖南汉族的遗传距离最小(0.005),与闽南汉族的遗传距离最大(0.035);在中国周边群体中,云南泸西汉族与新加坡华人的遗传距离最小(0.015),与日本人和马来西亚印度人群的遗传距离最大(0.060)。结果表明,17个Y-STR基因座在云南泸西汉族中具有较高的遗传多态性,适用当地的法医学应用。与其他民族群体的遗传多样性比较,对了解各群体的起源、迁徙及相互关系有重要意义。更多还原

【Abstract】 Seventeen human Y-chromosomal STR loci were selected to investigate the genetic polymorphisms of males in the Yunnan Luxi Han population,and to evaluate their forensic application values and genetic relationships with 16 other populations.The selected Y-STR markers included in the AmpFSTR Yfiler Amplification Kit were amplified in 156 unrelated Luxi Han males,and the PCR products were analyzed on the ABI Prism 3100 Genetic Analyzer.The AMOVA(analysis of molecular variance) method was applied to measure the genetic distance between populations.A total of 154 haplotypes were identified,of which 152 were unique.The observed haplotypes diversity value was 0.9998.The allele diversity values for each locus ranged from 0.3901(DYS437) to 0.9632(DYS385a/b).Among the domestic populations,the genetic distance between the Luxi Han and Hunan Han was the nearest(0.005),whereas the genetic distance between the Luxi Han and Minnan Han was the farthest(0.035).Compared with populations outside of China,the genetic distance between Luxi Han and the Singapore Chinese was the closest(0.015),whereas the genetic distance between the Luxi Han and Japanese and Malaysia Indians was the farthest(0.060).The combined Y-chromosome STR polymorphisms provide a powerful discrimination tool for forensic applications,and the study of genetic diversity among different populations is useful for the research of their origins,migrations and their interrelationships.更多还原