【摘要】 目的观察局部使用肿瘤坏死因子α反义寡核苷酸(TNF-α-ASON)对小鼠眼内单纯疱疹病毒(herpes simplex virus,HSV)-1感染的影响。方法将HSV-1注入小鼠前房建立动物模型。分别于建模前1d,建模后1d和4d结膜下注射TNF-α-ASON和PBS。8d和10d后观察两组小鼠眼部的炎症改变,测定病毒复制和眼内TNF-α含量。结果与对照组相比,8d时实验组葡萄膜炎发病率和眼内TNF-α含量显著降低(P<0.01),眼内HSV-1滴度无明显改变;10d时葡萄膜炎发病率和眼内HSV-1滴度都显著升高。结论局部使用TNF-α-ASON可以显著降低HSV-1感染小鼠眼内TNF-α含量,降低视网膜炎发病率。TNF-α在眼内具有抑制HSV-1复制的作用。更多还原

【Abstract】 Objective To investigate the effect of TNF-α antisense oligonucleotides treatment on subconjunctival herpes simplex virus(HSV)-1-induced uveitis.Methods HSV-1 was inoculated into the anterior chamber of mice.TNF-α-ASON and PBS were respectively injected subconjunctival into mice at-1 d,1 d and 4 d.The clinical course of retinitis,viral titer in the eyes and contents of TNF-α were analyzed.Results At day-8,TNF-α-ASON reduced the incidence of uveitis and the expression of TNF-α(P<0.01).The level of virus was not changed.However,the incidence of uveitis and viral titers were increased at day-10.Conclusions Topical TNF-α-ASON can reduce the contents of TNF-α and the incidence of uveitis.TNF-α may have an antiviral activity against HSV-1.更多还原