【摘要】 目的探究广州管圆线虫感染期幼虫(第Ⅲ期幼虫)在不同温度不同时间下的生存及死亡情况(死亡率)。方法采用人工消化法,从自然感染的褐云玛瑙螺中提取广州管圆线虫感染期幼虫,将其放入不同温度(35～90℃)的热水中,分别作用不同时间(1～20min),取出置解剖镜下观察虫体的变化情况。结果在1～20min内,45℃以下温度对感染期幼虫不具有杀灭作用,死亡率为0;50～55℃温度对感染期幼虫有轻度的杀灭作用,死亡率为12.00%～97.33%;60～65℃温度对感染期幼虫有一定强度的杀灭作用,死亡率为57.39%～100.00%;70～75℃温度对感染期幼虫有很强的杀灭作用,死亡率为97.22%～100.00%;在80℃以上温度时感染期幼虫迅速死亡,死亡率为100.00%。结论45℃以下温度,20min内对广州管圆线虫感染期幼虫没有杀灭作用;50～75℃温度,1～20min,对大多数广州管圆线虫感染期幼虫具有杀灭作用,温度越高,时间越长,杀灭作用越强;80℃以上温度,1～20min,对广州管圆线虫感染期幼虫具有完全杀灭作用。更多还原

【Abstract】 Objective To explore the survival and death (mortality) rate of infective larvae (No. Ⅲ larvae) of Angiostrongylus cantonensis under different temperatures and within different time periods. Methods Using artificial digestion method,infective larvae of Angiostrongylus cantonensis were collected from naturally infected Achatina fulica and were submerged into hot water at different temperatures (35～90℃) for different periods of time (1～20 min). Then the worms were removed from water to observe the anatomical changes under microscope. Results Blow 45℃ and between 1～20 min period, no killing effect was found and the mortality rate was 0; Between 50～55℃ a weak killing effect on the infective larvae was recorded, with the mortality rate 12.00%～97.33%; when temperature rose to 60～ 65℃ ,it showed a noticeable killing effect on the infective larvae with the mortality rate ranging from 57.39% to 100.00%. At 70～75℃ of temperature, killing effect was very strong, with the mortality rate at 97.22%～100.00%. And above 80℃ ,the infective larvae died quickly,the mortality rate was 100.00% .Conclusion Below 45℃ temperature, there is no killing effect for infective larvae of Angiostrongylus cantonensis in 20 min.There is a killing effect for the majority of infective larvae of Angiostrongylus cantonensis under 50 ～75℃ and within 1 ～20 min.The higher the temperature, the longer in the water, the stronger is the killing effect to infective larvae. Above 80℃, in 1～20 min, infective larvae of Angiostrongylus cantonensis will be killed completely.更多还原