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老虎须的组织培养与植株再生

Tissue Culture and Plant Regeneration of Tacca chantrieri Andre

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【作者】 张文珠林炳英林德钦

【Author】 ZHANG Wenzhu LIN Bingying LIN Deqin(National Plant Introduction Quarantine Base(Xiamen)/Xiamen Overseas Chinese Subtropical Plant Introduction Garden,Xiamen,Fujian 361002)

【机构】 厦门华侨亚热带植物引种园/国家农作物国外引种隔离检疫基地

【摘要】 以老虎须茎段为试验材料,采用不同激素组合对其进行愈伤组织诱导和分化、芽增殖与生根研究。结果表明:老虎须茎段愈伤组织诱导和分化的最适培养基分别为MS+6-BA 1.0 mg/L+KT 3.0 mg/L+NAA 0.5 mg/L和MS+6-BA 1.0 mg/L+KT 1.0 mg/L+NAA 0.5 mg/L;芽增殖的最适培养基为MS+6-BA 2.0 mg/L+IAA 0.2 mg/L;生根的最适培养基为1/2MS+IBA0.5mg/L。

【Abstract】 The callus induction and differentiation of Tacca chantrieri Andre and their shoots proliferation and rooting were studied using Tacca chantrieri Andre stems as the test material.The results showed that the best media for callus induction and differentiation of Tacca chantrieri Andre stem were MS+6-BA 1.0 mg/L+KT 3.0 mg/L+NAA 0.5 mg/L and MS+6-BA 1.0 mg/L+KT 1.0 mg/+NAA 0.5 mg/L respectively;the best medium for shoots proliferation was MS+6-BA 2.0 mg/L+IAA 0.2 mg/L;the best medium for rooting was 1/2 MS+IBA 0.5 mg/L.

【基金】 厦门市科技创新资金计划项目(NO.3502Z20102006)
  • 【文献出处】 热带农业科学 ,Chinese Journal of Tropical Agriculture , 编辑部邮箱 ,2010年12期
  • 【分类号】S567.239
  • 【被引频次】6
  • 【下载频次】126
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