【摘要】 目的探讨一氧化氮(NO)和血浆内皮素1(ET-1)在先天性心脏病(COHD)合并肺动脉高压中的作用及其临床意义。方法75例左向右分流型COHD患儿分为三组:其中无肺动脉高压组25例;轻度肺动脉高压组25例和中重度肺动脉高压组25例,正常对照组25例。采用放射免疫法和硝酸盐还原酶法测定血液中NO和ET-1含量,计算NO/ET-1比值。结果CHD组血浆ET-1水平(46.18±2.541 pg/m l)高于对照组(25.75±3.87 pg/m l),P<0.005;血清NO浓度(23.82±6.46μg/m l)低于对照组(37.27±2.47μg/m l),P<0.001;NO/ET-1的比值(0.68±0.39)明显低于对照组(1.18±2.80),P<0.001。CHD伴中重度肺动脉高压组的NO浓度低于轻度肺动脉高压组和无肺动脉高压组(P<0.001),肺动脉压力的程度和NO/ET-l的浓度呈负相关(r=-0.813,P<0.001)。结论COHD患者血浆ET-1浓度的升高和NO浓度的降低及NO/ET-1比值降低,在肺动脉高压形成和发展中起到了重要的作用。更多还原

【Abstract】 Objective To explore the function and clinic significance of NO and ET-1 in patients with COHD complicated with pulmonary hypertension(PH).Methods According to the degree of PH,75 cases with COHD were divided into 3 groups: none-PH group(25cases),mild PH group(25 cases) and mid-severe PH groups(24 cases).Meanwhile,other 25 normal cases were selected as the compared group.Measured the NO and ET-1 in blood by radioimmunoassy and nitrate reduction enzyme process.The ratio of NO/ET-1 was calculated.Results The ET-1 level of COHD group was 46.18±2.541 pg/ml,which is significantly higher than control group 25.75±3.87 pg/ml(P<0.005);the NO in COHD group 23.82±6.46 μg/ml was significantly lower than control group(37.27±2.47 μg/ml P<0.001).NO/ET-1 ratio in COHD group was 0.68±0.39,significantly lower than that of control group(1.18±2.80,P<0.001).The NO level of mid-severe PH group was significantly higher than that of none-PH group and mild-PH group(P<0.001).The pulmonary artery pressure was negatively correlated with the level of NO(r=-0.813,P<0.001).Conclusion It is play an important role in PH formation and development that increasing of ET-1 and decreasing of NO and the ratio of NO/ ET-1 in cases with COHD.更多还原