【摘要】 目的:探讨细胞角蛋白7(CK7)、细胞角蛋白20(CK20)在肝内胆管癌及肝内胆管结石旁胆管组织中的表达及临床意义。方法:免疫组织化学方法检测30例肝内胆管癌、例肝内胆管结石旁胆管组30织及15例正常肝内胆管组织中的CK7、CK20表达。结果:CK7在肝内胆管癌、正常肝内胆管组织中的阳性表达率分别为96.7%(29/30)、13.3%(2/15),差异有统计学意义(P<0.01);CK20在肝内胆管癌组织、正常肝内胆管组织中阳性表达率分别为43.3%(13/30)、(1/15),差异有统计学意义(P<0.01)。CK76.7%在肝内胆管结石旁胆管组织阳性表达率为76.6%(23/30),与正常肝内胆管组织阳性表达率13.3%(2/15)比较,差异有统计学意义(P<0.05)。CK20在肝内胆管结石旁胆管组织阳性表达率为6.7%(2/30),与正常肝内胆管组织阳性表达率6.7%(1/15)比较,差异无统计学意义(P>0.05)。结论:CK7和CK20可能成为肝内胆管癌特异性肿瘤标志物。更多还原

【Abstract】 Objective:To study the expression of CK7/CK20 in intrahepatic cholangiocarcinoma and hepatolithiasis.Methods:CK7/CK20 were analysed in 30 cases of intrahepatic changiocarcinoma and 30 cases of hepatolithiasis by immunohistochemical method.Results:The positive rates of CK7 expression were 96.7%(29/30)in intrahepatic cholangiocarcinoma and 13.3%(2/15)in normal intrahepatic bile duct respectively.Their difference was significant(P<0.01).The positive rates of CK20 expression were 43.3%(13/30)in intrahepatic cholangiocarcinoma,and 6.7%(1/15)in normal intrahepatic bile duct respectively.Their difference was significant(P<0.01).The positive rates of CK7 expression were 76.6%(23/30)in hepatolithiasis and 13.3%(2/15)normal intrahepatic bile ductrespectively.Their difference was significant(P<0.05).The positive rates of CK20 expression were 6.7%(2/30)in hepatolithiasis and 6.7%(1/15)in normal intrahepatic bile ductrespectively.Their difference was no significant(P>0.05).The expression of CK7/CK20 does not correlate with the sex and age of patients in intrahepatic changiocarcinoma and tumor’s histological grading(P>0.05).Conclusion:CK7/CK20 may be tumor makers of intrahepatic cholangiocarcinoma.更多还原