【摘要】 目的:比较低密度脂蛋白-阿克拉霉素(LDL-ACM)复合物和游离ACM对胃癌细胞株SGC-7901的抑制作用,从而为以LDL为载体介导的癌瘤靶向治疗提供理论支持。方法:37℃、CO25%及饱和湿度培养箱培养人上皮样胃癌细胞株SGC-7901及取自胃壁经原代培养获得的胃成纤维细胞。以LDL作为脂溶性抗肿瘤药物ACM的载体,温育交换法制备LDL-ACM复合物。观察LDL-ACM复合物及游离ACM对胃癌细胞株SGC-7901及胃成纤维细胞的增殖、细胞DNA合成及蛋白质合成的抑制作用,并进行统计学分析。结果:LDL-ACM复合物对胃癌细胞株SGC-7901的生长、DNA及蛋白质合成的抑制作用均显著强于游离ACM,且差异有统计学意义(P分别<0.01、0.05、0.005),且在药物浓度为1.2500～5.0000μg/mL范围内对于细胞DNA合成的抑制作用更显著(P<0.05)。而LDL-ACM复合物和游离ACM对正常胃成纤维细胞的生长、DNA的合成及蛋白质合成的抑制作用差异无统计学意义(P>0.05)。结论:以LDL为载体介导的肿瘤药物的细胞抑制作用强于游离抗肿瘤药物。更多还原

【Abstract】 Objective:To compare the inhibitory effects of low density lipoprotein-aclacinomycin(LDL-ACM)complex and free ACM on human gastric carcinoma cell line SGC-7901 and human normal gastric fibroblasts(control cell).Methods:LDL-ACM complex was obtained by incubating LDL with a large excess of ACM.Human gastric carcinoma cell line SGC-7901 and human normal gastric fibroblasts were obtained by purchase and breeding.The effects of LDL-ACM and free ACM on SGC-7901 cells were investigated,the inhibitory activity,the DNA synthesis inhibition and protein synthesis inhibition were observed.The data was statistically analyzed.Results:There was statistical significance between LDL-ACM and free ACM on SGC-7901 cell the inhibitory activity(P<0.01)and the DNA synthesis inhibitory effect(P<0.002),and at some drug concentration(1.25～5.00 μg/mL)there was obvious statistical significance(P< 0.05).The comparison of LDL-ACM complex and free ACM on human fibroblasts inhibitory activity and the DNA synthesis inhibitory effect showed no statistical significance(P>0.05).The LDL-ACM complex exerted a higher inhibitory effect of cell protein synthesis on SGC-7901 than free ACM(P<0.005).Conclusion:It can be included that the inhibitory effect of the complex mediated by LDL is greater than free drug,and therefore LDL may be used as a vehicle for drug targeting therapy to carcinoma cell.更多还原