【摘要】 该文设计并合成一种新的线性硝酮自由基捕获探针DSC-PBN(N-(4-(((2,5-dioxopy-rrolidin-1-yloxy)-carbonyloxy)methyl)benzylidene)-2-methylpropan-2-N-oxide),并采用UV、MS、1H NMR等一系列手段表征分子结构.进一步通过自由基捕获实验显示,DSC-PBN可与多种活泼自由基反应生成稳定的氮氧自由基加合物,且所得ESR谱图易于解析和分辨.由于DSC-PBN自由基捕获探针链接琥珀酰亚胺基团,可望通过与蛋白质(或多肽)N端的共价键合而应用于生物体系自由基分析.更多还原

【Abstract】 A novel linear nitrone spin-trap,DSC-PBN(N-(4-(((2,5-dioxopyrrolidin-1-yloxy)carbonyloxy)methyl)benzylidene)-2-methylpropan-2-N-oxide),was designed and synthesized.Its structure was characterized by UV,MS and 1H-NMR techniques.ESR results showed that the compound could be used to trap various free radicals efficiently,and the ESR spectra of the trapped radicals were readily recognizable.DSC-PBN could easily form a covalent link to the N-terminal peptides(or proteins) via succinimide intermediates,thus showing high potential for biological applications.更多还原