【摘要】 脂肪细胞决定和分化因子1(adipocyte determination and differentiation factor-1,ADD1)基因与人类和鼠的脂肪细胞分化具有确定的连锁关系。在脂肪细胞分化过程中,ADD1与转录因子PPARγ和C/EBP发挥着关键的作用。本研究克隆了保守DNA序列并用PCR-SSCP和直接测序法鉴定其多态性。同时,选择136头猪作为实验群体来探索其与肉质性状的关系。多态性分析发现:在第二外显子内一个导致错义突变的SNP,该SNP在实验群体内分布不平衡,未检测到AA基因型。相关分析结果显示,ADD1不同基因型间在肌内蛋白(MP)和肉色(MC)存在显著(P<0.05)的差异;肌内脂肪(IMF)存在极显著(P<0.01)的差异。说明,ADD1基因多态性有可能应用到提高肉质的育种实践中。更多还原

【Abstract】 Adipocyte determination and dfferentiation factor-1 gene (ADD1),a gene associated with adipocyte differentiation in the research of human and rat,plays a role during adipocyte differentiation with PPARγ and C/EBP.In this paper,we cloned a part of the gene sequence from pig,screened it with PCR-SSCP (single strand conformation polymorphism),and used sequencing to identify mutation.The result indicated that a SNP (single nucleotide polymorphism) happened in the conservative domain of the ADD1 gene,which led to a different amino-acid sequence and a new site for proteinase.To verity the relationship between this mutation and meat quality,we chose 136 pigs as the experimental population,all of which were butchered to determine meat quality.The result of polymorphism analysis showed that a novel SNP was found in second exon.The polymorphism was disequilibrium in population and AA genotype was not found in it.The relation analysis showed that intramuscular protein,intramuscular fat and meat color had obviously difference among different ADD1 genotypes of pigs.The ADD1 polymorphism can apply to pig breeding for improving meat quality.更多还原