【摘要】 为了检验发情牛(Bos taurus)血清在猪卵母细胞成熟中的应用,实验分别在成熟培养液中加入不同的血清,观察卵母细胞的成熟效果,并通过孤雌激活胚胎和重构胚的体外发育能力来判断血清对卵母细胞质量的影响;研究还进行了用发情牛血清成熟的卵母细胞构建的核移植胚胎的体内发育试验。结果表明,发情牛血清,成年牛血清和胎牛血清在猪卵母细胞的成熟率方面没有显著差异(P>0.05);卵母细胞成熟时加入不同的血清,对卵母细胞体外发育能力的影响不显著(P>0.05);来自含发情牛血清成熟液培养的卵母细胞,与胎儿成纤维细胞构建的克隆胚胎能够完成全期发育。更多还原

【Abstract】 In order to determine the effect of estrous cow(Bos taurus) serum on porcine oocyte maturation,various sera was added to maturation medium and the matured results of oocyte were observed.The effect of serum on oocyte quality was tested by in vitro developmental abilities of parthenactivation and reconstructed embryo.Moreover,in vivo development ability of nuclear transfer embryos constructed by oocytes matured with medium supplemented estrous cow serum was tested.Results indicated that there was no significant difference in rate of maturation among three sera(estrous cow serum,cow serum,and fetal cow serum) supplementation medium(P> 0.05).In vitro developmental capacities of oocyte matured in medium supplemented various sera were not signifi cantly different(P>0.05).Embryos reconstructed with fetal fibroblast cell and oocyte derived from estrous cow serum medium could complete full-term development.更多还原