【摘要】 目的:通过一期门静脉缩窄法制备犬门静脉高压症动物模型,观察门静脉压力的变化情况,探讨制备动物模型时门静脉压力控制的合理范围。方法:犬24只,分为A、B、C3组,行开腹结扎法缩窄门静脉,术中A组门静脉压力控制在20.0～25.0cmH2O,B组控制在25.0～30.0cmH2O,C组控制在30.0～35.0cmH2O。分别于术后2、4、6、8、10、12、14周测量门静脉压力。并于14周开腹观察胃底食管下段静脉曲张情况。结果:术后各组门静脉压力逐渐下降,14周门静脉压力已平稳。A组门静脉压力自(22.87±1.43)cmH2O下降至(19.56±0.80)cmH2O(P<0.05),14周后有4只动物胃底食管下段静脉曲张不显著,无动物死亡。B组门静脉压力自(27.22±1.47)cmH2O下降至(21.81±0.99)cmH2O(P<0.05),14周后所有动物胃底食管下段静脉曲张显著,1只动物死亡;C组门静脉压力自(31.86±1.52)cmH2O下降至(25.32±1.79)cmH2O(P<0.05),所有动物胃底食管下段静脉曲张显著,3只动物死亡。结论:一期门静脉缩窄法制备犬门静脉高压症动物模型,术中门静脉缩窄后门静脉压力应控制在25.0～30.0cmH2O,在这个范围内动物模型胃底食管下段静脉曲张显著,且死亡率低。更多还原

【Abstract】 Objective:To study a reasonable range on controlling portal vein pressure through the change of portal vein pressure in the process of establishing portal hypertension animal models in canines by partial portal vein ligation.Methods:Twenty four canines were divided three groups randomly,and every group had six cases.Ligations of the portal vein were performed in all canines.Intraoperatively the portal vein pressure was controlled in a range of 20.0～25.0 cmH2O in group A and 25.0～30.0 cmH2O in group B and 30.0～35.0 cmH2O in group C.The portal vein pressure was measured after 2 weeks,4 weeks,6 weeks,8 weeks,10 weeks,12 weeks and 14 weeks.The condition of esophageal varices gastric was observed after 14 weeks.Results:Portal vein pressure descended gradually in each group,and portal vein pressure was smooth after 14 weeks.In group A the portal vein pressure decreased from (22.87±1.43) cmH2O to (19.56±0.80) cmH2O (P<0.05) after 14 weeks,and significant esophageal varices was not appeared in four canines,and no animals died;In group B the portal vein pressure decreased from (27.22±1.47) cmH2O to (21.81±0.99) cmH2O (P<0.05) after 14 weeks,and significant esophageal varices was appeared in all canines,and one animal died;In group C the portal vein pressure decreased from (31.86±1.52) cmH2O decreased to (25.32±1.79) cmH2O (P<0.05),and significant esophageal varices was appeared in all canines,and three animals died.Conclusions:In the process of establishing portal hypertension models in canines by partial portal vein ligation,the portal venous pressure should be controlled in a range of 25.0～30.0 cmH2O,in this range the esophageal varices of animal models was significant and death rate was lower.The portal hypertension animal models in canine can be used in a variety of experimental studies.更多还原