【摘要】 目的:研究驱动蛋白家族成员11(kinesin family member 11,Kif11)和驱动蛋白家族成员15(kinesin familymember 15,Kif15)在大鼠失神经肌萎缩及再支配过程中的表达变化。方法:建立大鼠坐骨神经夹伤模型,测定腓肠肌湿重比了解肌萎缩程度,采用足迹实验测定坐骨神经功能指数了解神经损伤再支配恢复情况,采用Real-time PCR方法检测坐骨神经夹伤后不同时间腓肠肌组织中Kif11和Kif15 mRNA水平的表达变化。结果:在神经夹伤早期(1～7 d),腓肠肌组织中的Kif11和Kif15 mRNA迅速上升,7～14 d达到高峰,随后mRNA水平逐渐下降,至28 d时基本接近正常表达水平;同时可观察到腓肠肌湿重比及坐骨神经功能指数的恢复。结论:Kif11和Kif15 mRNA的变化与坐骨神经损伤(夹伤)引起的肌萎缩程度呈正相关。更多还原

【Abstract】 Objective: To investigate the expression pattern of kinesin family member 11(Kif11) and kinesin family member 15(Kif15) in rat gastrocnemius after sciatic nerve crushed.Methods: The Kif11 and Kif15 mRNA were conducted by quantitative real-time PCR.The ratio of muscle wet weight and sciatic functional index(SFI) were measured.Results: The expression levels of Kif11 and Kif15 mRNA in gastrocnemius were increased instantly in the early stage(1～7 d) after sciatic nerve crushed.Then,they reached to the peak from 7 d to 14 d,followed by decreased gradually after 14 d and closed to the normal expression levels at 28 d.At the same time,the recovery of the ratio of muscle wet weight and SFI was observed.Conclusion: The results of this study provided evidence for a significant positive correlation between Kif11,Kif15 expression and muscle atrophy within a 28-day period after nerve injury.This suggested that Kif11 and Kif15 might play an important role in the rat gastrocnemius atrophy and regeneration.更多还原