【摘要】 近年来,随着微操作研究的不断发展和深入,批量目标微操作成为当前的研究热点之一.由于显微镜视野范围狭小,微操作过程中需要不断进行物镜转换和调焦工作.本文研制的全自动微操作平台将调焦和物镜转换改造为电动方式,实现了自动调焦和物镜自动转换功能.针对细胞图像的特点,本文提出了基于扫描线的归一化方差聚焦测度方法,并在此基础上,设计了相应的聚焦平面搜索策略.针对显微镜物镜转化器的特点,本文设计了自动物镜转换结构和控制方法,实现不同放大倍数物镜的转换.最后本文在细胞图像处理的实验中,构建了具有分层递阶结构的全局视野,验证了自动调焦和物镜自动转换的准确控制,为批量目标操作的自动化奠定了基础.更多还原

【Abstract】 With the development of micro-manipulation techniques,batch-targets-oriented operation has become the focus in the research. During batch-targets-oriented micro-manipulation,the objective lens and focused position need to be adjusted from time to time because the view field of microscope is very limited. This paper introduced an automated micro-manipulation platform with auto-focusing and autoswitching of the objective lens to realize batch-targets-oriented manipulation. An improved normalized variance algorithm based on scanning lines was presented to save time for online calculation. A searching strategy based on the algorithm was designed to realize the auto-focusing under any condition. A set of special mechanism of auto-switching of the objective lens was introduced with the control method. Finally,in the experiment of cell image processing,the global field of view ( FOV) of the hierarchical structure was constructed,which is the foundation of batch-targets-oriented micro-manipulation. The test result verifies the validity and correctness of the improvement in the microscope’s visual system.更多还原