【摘要】 研究大黄酸对IL-2引起大鼠T淋巴细胞增殖和细胞内[Ca²⁺]_i变化的影响.采用MTT法检测细胞增殖,用单细胞钙成像系统记录胞内游离Ca²⁺浓度([Ca²⁺]_i).结果表明,大黄酸对IL-2引起的细胞增殖有显著抑制作用,且抑制效果与剂量有关;IL-2引起[Ca²⁺]_i升高,并持续维持高[Ca²⁺]_i水平,[Ca²⁺]_i升高的机制包括胞内Ca²⁺释放和胞外Ca²⁺内流;大黄酸显著抑制IL-2引起的[Ca²⁺]_i升高.结果提示大黄酸可抑制T淋巴细胞增殖,作用机制可能与抑制细胞内[Ca²⁺]_i升高相关.更多还原

【Abstract】 The aim of this study is to investigate the effect of rehein on IL-2 induced proliferation and intracellular Ca²⁺ mobilization of T lymphocytes.The proliferation of T cell was tested by MTT assay.The intracellular Ca²⁺ mobilization was recorded by single cell Ca²⁺ image system using Fura-2 as Ca²⁺ probe.Rehein inhibited IL-2 induced proliferation with a dosedependent manner.IL-2 triggered a rapid intracellular Ca²⁺ rise followed by a sustain high intracellular Ca²⁺ level.The rapid Ca²⁺ rise was caused by Ca²⁺ release from intracellular store and the sustain high intracellular Ca²⁺ level was due to Ca²⁺ influx.Rehein inhibited both Ca²⁺ release and Ca²⁺ influx.These results suggested that Rehein blocked IL-2 induced proliferation of T lymphocytes,and the mechanism for this may be due to its inhibition on Ca²⁺ mobilization.更多还原