【摘要】 目的:探讨在急性胰腺炎(AP)大鼠血清中抵抗素、C反应蛋白(CRP)、白介素1-β(IL-1β)、肿瘤坏死因子α(TNF-α)水平的变化。方法:用ELISA测定大鼠血清中抵抗素、TNF-α、IL-1β、CRP水平变化,同时检测胰腺/体重比值、胰腺组织病理变化。结果:急性水肿型胰腺炎(AEP)组和急性坏死型胰腺炎(ANP)组大鼠血清淀粉酶(AMY)、抵抗素、TNF-α、IL-1β、CRP水平较正常对照组、假手术组均明显升高,ANP组与AEP组相比升高更明显,两者之间差异显著(P<0.01或者P<0.05)。血清抵抗素水平与血清TNF-α、IL-1β、CRP水平和胰腺组织病理评分呈正相关性,血清CRP水平和胰腺组织病理评分也呈正相关性。结论:抵抗素可能与急性胰腺炎的、发生、发展有关,是评估急性胰腺炎严重程度的有价值指标之一。更多还原

【Abstract】 Objective:To investigate the variety of resistin,CRP,TNF-α and IL-1β in the rat of acute pancreatitis.Methods:Forty Sprague-Dawley rats were randomly divided into four groups:control group,sham operation group,AEP group and ANP group.AEP model was induced by intraperitoneal injection of cerulein,and ANP model was established by intraperitoneal injection of L-arginine while the control group didn’t receive any interference and the sham opertation group received same volume injections of 0.9% saline solution.The level of amylase,resistin,CRP,TNF-α and IL-1β were determined in blood serum by using ELISA,and pancreas /body weight ratio was evaluated and pancreatic pathology was recorded.The level of resistin was detected in pancreas by immunohistochemistry.Results:The levels of amylase,pancreas /body weight ratio,pancreatic pathology,resistin,TNF-α,IL-1β and CRP were respectively(4376.70±342.95)U/L,(8.67±1.43)g/kg,5.39±0.26,(10.21±3.34)ng/ml,(184.18±45.24)pg/ml,(194.24±44.81)pg/ml,(3585.85±157.28)ng/ml in AEP group;and were respectively(6750.2±321.81)U/L,(9.33±1.76)g/kg,7.81±0.28,(15.14±3.11)ng/ml,(349.31±94.54)pg /ml,(315.59±37.04)pg /ml,(4345.04±661.53)ng /ml in ANP group.All the indexes were significantly higher than those in the control group and the sham operation group(P < 0.01),the levels of the indexes in ANP group were higher than those in AEP group(P < 0.01 or P < 0.05).Resistin was correlated with the increase of CRP,TNF-α,IL-1β and the pathologic severitity of pancreatitis,respectively,(r=0.711,0.812,0.794,0.812,P < 0.01).The pathologic severitity of pancreatitis was correlated with the level of CRP,TNF-α,IL-1β.Conclusion:The resistin is correlated with the pathogenesis and development of acute pancreatitis,and may be useful to predict the severity and prognosis of acute pancreatitis.更多还原