【摘要】 目的:对比改进的"天花板"组织块培养法与消化培养法培养前脂肪细胞的优劣。方法:两种方法获得的前脂肪细胞,培养3天,显微镜下检测自分化情况,MTT、流式细胞仪检测其增殖情况和细胞周期。培养9天,绘制生长曲线。使用含胰岛素、甲状腺素T3和转铁蛋白(ITT)的无血清培养基诱导获得的前脂肪细胞5天,拍照测细胞直径,油红染色法检测其分化情况。结果:改进的"天花板"组织块培养法培养的第一代自分化存在,但是发生自分化的细胞很少。增殖和分化结果均无显著性差异。但是此法在同等情况下获得细胞较多,所需经费较少。结论:与消化培养法相比较而言,改进的"天花板"组织块培养法是一种更加简单、经济的获得大鼠前脂肪细胞的方法。更多还原

【Abstract】 Objective:To compare improved ‘ceiling’tissue culture method with digestion culture method to choose one method which can culture preadipocytes more properly. Methods:Cells obtained by these two methods,cultured 3 days,were determined by microscope for their self-differentiation. Preadipocytes proliferation and cell cycle were measured by MTT spectrophotometry and flow cytometer. After cultured 9 days,cells were counted to draw growth curves. After induced by DMEM / F12 with insulin,thyrine and transterrin (ITT) 5 days,the diameters of cells were determined by Image-Pro plus5.0 software and the content of lipid was measured by Oil Red O staining. Results:A few adipocytes were obtained by ‘ceiling’tissue culture method appeared in the first generation cells. The results of proliferation and differentiation are similar in the cells obtained by the two methods. But ‘ceiling’tissue culture method can obtain more preadipocytes and need less money than digestion culture method. Conclusion:Comared with digestion culture method,improved‘ceiling’tissue culture method is a more simple and economic method which can obtained more preadipocytes更多还原