【摘要】 为了验证营养体不亲和性导致细胞凋亡的假说,对栗疫病菌融合细胞基因组DNA的降解物进行了琼脂糖凝胶电泳检测,观察了融合细胞的细胞核变化,测定了融合细胞的活性氧产生、脂肪粒积累以及进行了融合细胞死活鉴定。结果表明:不同亲和群菌株的孢子混合涂板,孵育后经历萌发、细胞融合,融合细胞基因组DNA经琼脂糖凝胶电泳可以检测到DNA梯状条带,这一特征为营养体不亲和性诱导的程序性细胞死亡提供了新的证据;细胞学上可观察到不亲和性菌株融合细胞细胞核降解、活性氧产生减少、脂肪粒积累增多及融合细胞死亡,这些特征与细胞凋亡的特征相似。更多还原

【Abstract】 To test the hypothesis whether vegetative incompatibility results in nuclear degradation,a characteristic of apoptosis,we mainly use the agar electrophoresis detection of the degeneration of fusion cell genome DNA of chestnut blight fungus Cryphonectria parasitica,the cytological observation on the change of the nuclear in hyphae between incompatible strains,the production of the reactive oxygen species,the accumulation of lipid droplet and the identification of the live and dead cell.The results showed that,the electrophoresis detected the DNA ladder,and this characteristics gives the new evidence of vegetative incompatibility induced cell death.Hyphal fusion cells between vegetative incompatible strains were compartmentalized by septal plugging and contained heavily degraded nuclear DNA.Reactive oxygen species accumulation decreased in hyphal fusion cells,the accumulation of lipid droplets increased and finally the hyphal fusion cell died.The morphological features of hyphal compartmentation and death during vegetative incompatibility can be described as a form of programmed cell death.更多还原