【摘要】 研究液体摇瓶发酵产纤维素酶的最佳培养基组成。通过单因素实验确定了培养基中氮源、碳源和诱导碳源的种类,采用正交试验确定了培养基中4种主要营养成分的组成。结果表明,摇瓶液体发酵产纤维素酶的最佳产酶培养基的种类和组成为:有机氮源蛋白胨和无机氮源硫酸铵的含量分别为11 g/L和13 g/L,葡萄糖6 g/L,磷酸二氢钾10.5 g/L,爆破的稻草为13.4 g/L;纤维素酶的滤纸酶活为19.22 U/mL。更多还原

【Abstract】 The best culture medium for the preparation of cellulase by liquid fermentation of Trihoderma viride was determined in this paper.Firstly,the species of nitrogen source,carbon source and induced carbon source were determined through single factor test.Then four main compositions of culture medium were determined through orthogonal experiments.The results showed that the compositions of the best culture medium for cellulase preparation were as follows: peptone content(organic nitrogen source) was 11 g/L,(NH4)2SO4 content(inorganic nitrogen source) was 13 g/L,glucose content was 6 g/L,KH2PO4 content was 10.5 g/L,and steam-explosion straw content was 13.4 g/L.The filter paper activity of the produced cellulase was 19.22 U/mL.更多还原