【摘要】 采用含有4种菌的菌剂与多种有机肥联合修复石油污染土壤,通过盆栽实验对不同浓度菌剂处理土壤中的石油烃降解率、16种多环芳烃(PAHs)浓度、脱氢酶活性、pH、阳离子交换量和微生物多样性等变化进行了研究。结果表明,腐植酸、诺沃肥和生物有机钙等有机肥和菌剂(4%处理)的加入使土壤盐碱环境得到明显改善,土壤pH稳定于6.9,阳离子交换量为201.94cmol·kg-1;对比4个不同浓度菌剂处理的效果,4%菌剂处理与有机肥联合作用修复效果最显著,石油烃降解率可达到73%,大部分所测PAHs浓度显著降低,其中萘、蒽、苯并(a)芘和苯并(g,h,i)芘降解率分别达到了65.5%、57.7%、74.7%和55.5%,土壤微生物数量增加,多样性更为丰富。更多还原

【Abstract】 In this study,bioremediation of petroleum-contaminated soil was investigated using 4 mixed cultures and organic fertilizer.The degradation efficiency,the concentrations of the 16 priority PAHs,the dehydrogenase activity,pH,the cation exchange capacity(CEC)and microbial diversity of the soil were monitored for different treatments in pot experiments.The results showed that the addition of organic fertilize(rhumic acid,NovoGro and biological organic calcium)and mixed microbes(4% addition of mixed cultures)notably improved the futility of the saline soil.The pH of the treated soil was stable at 6.9 and CEC increased to 201.94 cmol·kg-1.Combination of the 4% microbial consortium and organic fertilizer provided the greatest degradation efficiency.With this treatment,the total concentration of petroleum hydrocarbon decreased by 73% and most of the PAHs decreased significantly.The degradation percentages of naphthalene,anthracene,benzo(a)pyrene and benzo(g,h,i)perycene were 65.5%,57.7%,74.7% and 55.5%,respectively.Both the abundance and diversity of soil bacteria were stimulated during the bioremediation process.更多还原