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鄱阳湖中华鳖染色体核型、G带和Ag-NORs的分析

Analysis on Karyotype,G-Band and Ag-NORs of Chromosome of Turtle,Trionyx s.Sinensis from Poyang Lake

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【作者】 王军花; 饶友生; 龚贵如; 洪一江;

【Author】 WANG Jun-hua1,RAO You-sheng1,2,GONG Gui-ru1,HONG Yi-jiang1(1.Department of Bioscience,Nanchang University,Nanchang 330031,China;2.Biological Department of Jiangxi Educational College,Nanchang 330029,China)

【机构】 南昌大学生物科学系; 江西教育学院生物技术研究所;

【摘要】 以常规方法制备鄱阳湖中华鳖的染色体标本,分析其核型。其核型公式为2n=66=4M+8Sm+16T+38mc,NF=78。用胰酶法制备染色体G-带,绘制了G带模式图。采用了稍加修改的Foresti方法研究鄱阳湖中华鳖染色体的Ag-NORs核型,其Ag-NORs数目主要为2个,多态性主要表现为银染粒的大小和染色的深浅,数目的多态不显著。比较鄱阳湖中华鳖和其它一些已研究过的龟鳖类动物的银染核型,绝大多数种类的Ag-NORs数为2,这说明龟鳖类动物rDNA非常保守,该类动物的演化过程较为缓慢。

【Abstract】 The chromosome preparation of turtle,Trionyx s.sinensis from Poyang Lake was made by the normal air-dried method.The karyotype of the turtle is 2n=66=4M+8Sm+16T+38mc,NF=78.The G-banding of chromosomes were made by the trypsinase method,and the schema chart of it was mapped out.The Ag-NORs was studied with the improved Foresti method.The number of Ag-NORs is 2 in its chromosome.Results indicate that the rDNA is very conservative,and the evolution process of this animal was slowness.

【关键词】 中华鳖; 核型; G-带; Ag-NORs; 鄱阳湖;
【Key words】 turtle(Trionyx sinensis); karyotype; G-banding; Ag-NORs; Poyang Lake;
【基金】 江西省科技支撑基金资助项目(2010)
  • 【文献出处】 南昌大学学报(理科版) ,Journal of Nanchang University(Natural Science) , 编辑部邮箱 ,2010年05期
  • 【分类号】Q953
  • 【被引频次】6
  • 【下载频次】229
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