【摘要】 以白介素-1β(IL-1β)诱导的人肺癌细胞A549为模型,探讨原花青素(PC)对环氧合酶-2(COX-2)启动子活性的影响。用含有完整和NF-κB结合位点突变的COX-2启动子的的荧光素酶表达载体pGL 3质粒,转染A549细胞,加原花青素(20 mg/L)培养,检测COX-2基因5’调控区相对转录活性;RT-PCR检测细胞COX-2 mRNA的表达。结果发现NF-κB结合位点突变的COX-2基因转录活性极大地降低,原花青素可以显著降低完整的COX-2启动子转录活性,降低A549细胞中COX-2 mRNA的表达。更多还原

【Abstract】 Transfect the normal and the NF-κB mutant plasmids to the A549 cells to determine the activity of the COX-2 promoter in IL-1β-induced A549 cells,detect the effect of proanthocyanidins on the expression of COX-2 mRNA in IL-1β-induced A549 cells by RT-PCR.The result is NF-κB mutant COX-2 promoter has little activity;proanthocyanidins inhibited the activity of COX-2 promoter and inhibited the expression of COX-2 mRNA.更多还原