【摘要】 为检测普通小麦D染色体组是否存在psy基因,以扩增普通小麦(2X=AABBDD=42)psy基因的相同引物psy02和psy06在节节麦(2X=DD=14)中进行PCR反应。结果表明,引物psy02在节节麦基因组DNA中的扩增产物长206 bp,与普通小麦中扩增的196 bp序列同源率为93.0%,对应的第2外显子区域内仅有1 SNP;引物psy06在节节麦基因组DNA中的PCR产物长305 bp,与普通小麦中扩增的302 bp序列同源率达95.77%,对应的第6外显子区域内无SNP,说明在小麦D染色体组中存在psy基因,且psy基因部分外显子序列在D染色体组的进化过程中相对保守。更多还原

【Abstract】 In order to verify whether there is psy gene on the DD genome of Triticum aestivum or not, the same primers,psy02 and psy06,were used to amplify psy gene in Triticum tauschii(2X = DD = 14) and Triticum aestivum(2X= AABBDD= 42).The results showed that a 206 bp DNA fragment was amplified by the primers psy02 in Triticum tauschii and a 196 bp DNA fragment was amplified in Triticum aestivum,sequences analysis displayed the homology of the two DNA fragment was 93.0%, and there was only one SNP in the 2nd exon region of psy gene;To the primers psy06,a 305 bp DNA fragment was amplified in Triticum tauschii,a 302 bp DNA fragment in Triticum aestivum,the homology of the two fragment was 95.77%,and there was no SNP in the 6th exon area of psy gene, these results indicated that there was psy gene on the DD genome of Triticum aestivum,and some ex-ons sequence of psy gene in the DD genome were relatively conservative in Triticum tauschii and Triticum aestivum.更多还原