【摘要】 通过抑制消减杂交技术成功地构建了亚洲棉石系亚1号耐旱相关基因cDNA文库。通过蓝白斑筛选、菌落PCR及生物信息学等分子生物学手段分析和检验了库的质量,最终筛选出阳性克隆392个。通过EST测序得到265个单一序列,其中41个为重叠群,224个为单拷贝。生物信息学分析结果表明,该文库含有大量与耐旱相关的基因,并且涉及植物生理中多种代谢途径。更多还原

【Abstract】 This study constructed high quality drought-related cDNA library of cotton (Gossypium arboreum L.) by SSH successfully;it was checked and analyzed by Blue-white bolting,colony PCR,and bioinformatics.Finally,392 positive clones were obtained.After cluster analysis of the ESTs sequences,265 unigenes were obtained,including 41 contigs and 224 singlets.A large group of drought stress-induced genes were found in the cDNA library,which involved in many metabolism pathways.更多还原