【摘要】 目的探讨趋化因子CXCL12及其受体CXCR4在正常脑组织和各级星形细胞肿瘤中的表达及其与临床病理特征的关系。方法采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结法(streptavidin-perosidase,SP)和半定量逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)测定5例正常脑组织和61例Ⅱ级、Ⅲ级星形细胞瘤组织中的CXCL12和CXCR4蛋白和mRNA表达。结果正常脑组织中,CXCL12仅在神经元细胞呈弱阳性表达,CXCR4无表达;CXCL12在Ⅱ级、Ⅲ级星形细胞瘤中的阳性率分别为32.1%和75.7%,表达量(IOD)为0.61±0.07和1.13±0.12;CXCR4在Ⅱ级、Ⅲ级星形细胞瘤中的阳性率分别为35.7%和72.7%,表达量(IOD)为0.39±0.06和1.06±0.12,两组比较均有显著差异(P<0.05)。结论 CXCL12和CXCR4在星形细胞瘤中常为共表达,并随病理分级的增高阳性率和表达量明显增加,表明CXCR4可能与肿瘤的恶性生物学行为有关,有望成为治疗星形细胞肿瘤的新途径。更多还原

【Abstract】 Objective To investigate the expression of chemokine CXCL12 and its receptor CXCR4 in normal brain and astrocytomas(grade Ⅱ-Ⅲ)and its correlation with clinical pathology features.Methods CXCL12 and CXCR4 protein and mRNA were detected in 5 normal brain and 61 astrocytomas by immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).Results In normal brain fissues,low levels of CXCL12(weak positive)were observed in neurons and CXCR4 expressions were negative.In grade Ⅱ and Ⅲ astrocy toma fissue,the positive rate of CXCL12 protein was 32.1% and 75.7% while CXCL12 mRNA was 0.61±0.07 and 1.13±0.12,respectively.The positive rate of CXCR4 protein was 35.7% and 72.7% while CXCR4 mRNA was 0.39±0.06 and 1.06±0.12,respectively.There is significant difference between grade Ⅱ and Ⅲ astrocytoma(P<0.05).Conclusion In astrocytoma tumors,CXCL12 and CXCR4 expressions are colocalized when both are expressed.CXCL12 and CXCR4 expressions increas with increasing tumor pathologic grades.It suggests that CXCR4 may be related to malignant behavior of tumor cells and may provide a new target for therapy.更多还原