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不同株系拟松材线虫对黄山松和黑松苗木的致病性
Pathogenicity of Different Isolates of Bursaphelenchus mucronatus to Pinus taiwanensis and P. thunbergii Seedlings
【摘要】 采用23个不同来源的拟松材线虫株系对2年生黄山松和2年生黑松进行人工接种试验。结果表明:1)对黄山松苗具有致病作用的拟松材线虫有4个株系,分别为CFS4,SLD3,ZJ1和KOR1。其中接种ZJ1,SLD3 2个株系的黄山松苗的死亡率均为16.7%。2)对黑松具有致病作用的拟松材线虫有9个株系,分别是CFL1,SFS1,SFS4,SLD9,SDZ1,ANL5,ANL7,AHS9和HYC1。感病指数最高的ANL5达到83.3。其次是AL7,感病指数为54.2。发病率最高的拟松材线虫株系同样是ANL5,发病率达100%。死亡率最高的拟松材线虫系是ANL5和ANL7,达50%。3)根据拟松材线虫对2年生黑松苗的感病指数、发病率、死亡率等指标,将供试的23个拟松材线虫株系划分为3个致病等级:Ⅰ级(强致病性):ANL5和ANL7个株系;Ⅱ级(弱致病性):CKL1,SFS1,SFS4,SLD9,SDZ1,AHS9和HYC1 7个株系;Ⅲ级(无致病性):ANL1,JLH1,JLH10,JXY7,JYX31,CFL4,SLD3,YWF1,GBL3,ZJ1,HN1,TW1,JAP1和KOR1 14个株系。4)来自安徽的4个拟松材线虫株系,有3个株系对2年生黑松苗具有致病作用,其中2个株系(ANL5,ANL7)具有强致病作用;来自四川的5个拟松材线虫株系,有4个株系(SFS1,SFS4,SLD9,SDZ1)对2年生黑松苗具有中等强度的致病作用,而另外1个株系(SLD3)对2年生的黄山松苗具有致病作用;来自江苏的4个拟松材线虫株系(JLH1,JLH10,JXY7,JYX3)对2年生黄山松苗、2年生黑松苗均无致病性。
【Abstract】 Twenty-three strains of Bursaphelenchus mucronatus were used for inoculating two-year-old seedlings the following of Pinus taiwanensis and Pinus thunbergii to investigate their pathogenicity in this study.The main results were: 1)Four of Twenty-three B.mucronatus isolates had pathogenicity on the two-year-ole seedlings of P.taiwanensis,and they were SFS4,ZJ1,KOR1 and SLD3 respectively.2) Nine of Twenty-three isolates of B.mucronatus had pathogenicity on the two-year-old seedlings of P.thunbergii,and they were CFL1,SFS1,SFS4,SLD9,SDZ1,ANL5,ANL7,AHS9 and HYC1 respectively.3) According to disease index,morbidity rate and disease rate of B.mucronatus to two-year-old seedlings of P.thunbergii,twenty-three isolates of B.mucronatus were divided into three levels: Class Ⅰ(strong pathogenicity),Class Ⅱ(weak pathogenicity) and Class Ⅲ(no pathogenicity).4) Three of four populations from Anhui province had pathogenicity to two-year-old seedlings of P.thunbergii,wmong which two populations(ANL5 and ANL7) had strong pathogenicity;Four(SFS1,SFS4,SLD9,SDZ1) of five populations from Sichuan province had pathogenicity to two-year-old seedlings of P.thunbergii,and another population(SLD3) had pathogenicity to two-year-old seedlings of P.taiwanensis;All four populations from Jiangsu province had no pathogenicity to two-year-old seedlings of P.thunbergii and P.taiwanensis
【Key words】 Bursaphlenchus mucronatus; pathogenicity; Pinus taiwanensis; Pinus thunbergii;
- 【文献出处】 林业科学 ,Scientia Silvae Sinicae , 编辑部邮箱 ,2010年12期
- 【分类号】S763.18
- 【被引频次】30
- 【下载频次】359