节点文献
百合MDHAR基因的克隆与表达分析
Cloning and Expression Analysis of MDHAR from Lilium longiflorum
【摘要】 <正>抗坏血酸(ascorbic acid,AsA)作为植物细胞内重要的自由基清除剂,是通过参与一系列的氧化还原反应而发挥抗氧化剂的功能。单脱氢抗坏血酸还原酶(monodehydroascorbate reductase,MDHAR)在抗
【Abstract】 A all-length cDNA of LlMDHAR gene was cloned from Lilium longiflorum cv.’White Heaven’ by RACE.The gene consisted of 1 502 bp encoding a protein of 434 amino acids.The sequencing indicated that the LlMDHAR gene shared highly homology with other plants.RT-PCR analysis showed that expression of LlMDHAR gene could be detected in the root,bulb and leaf,and the relative high expression occurred in the root and leaf.Under H2O2 stress,the expression of LlMDHAR gene was increased with the raising of H2O2 concentration.
【关键词】 铁炮百合;
MDHAR;
克隆;
表达;
【Key words】 Lilium longiflorum; monodehydroascorbate reductase gene(MDHAR); cloning; expression;
【Key words】 Lilium longiflorum; monodehydroascorbate reductase gene(MDHAR); cloning; expression;
【基金】 国家教委博士点基金(200800190014)
- 【文献出处】 林业科学 ,Scientia Silvae Sinicae , 编辑部邮箱 ,2010年09期
- 【分类号】S682.29
- 【被引频次】15
- 【下载频次】256