【摘要】 目的腹主动脉缩窄大鼠心力衰竭（心衰）模型通过腹腔给予硫化氢（H₂S）供体硫氢化钠（NaHS）,观察压力负荷性心衰形成过程中H₂S对氧化应激状态的影响。方法 SD大鼠63只,随机分为对照组、心衰组、NaHS组,每组21只。各组均在第4、8、12周时,各取7只大鼠取血及心脏进行检测。结果心衰组4、8、12周血清H₂S含量、心肌H₂S合酶活性均较对照组明显降低,NaHS组4、8、12周血清H₂S含量较心衰组明显升高,心肌H₂S合酶活性较心衰组略有增高,但仍低于对照组。与对照组比较,心衰组4、8、12周血清MDA含量明显升高,超氧化物歧化酶（SOD）活性明显降低;与心衰组比较,NaHS组SOD活性上升,MDA含量下降,其中MDA含量在8、12周时下降明显,但仍明显高于对照组;SOD在12周时明显增高,但仍明显低于对照组。与心衰组比较,NaHS组4、8、12周血清氧化型低密度脂蛋白含量明显降低,但8、12周时仍明显高于对照组。结论外源性给予H₂S供体NaHS能改善压力负荷引起的心衰大鼠体内和心肌内的氧化应激状态,可能是H₂S心脏保护作用机制之一。更多还原

【Abstract】 Objective To observe the effects of H₂S on oxidative stress in heart failure induced by pressure-overload in rats.Methods Sixty-three male SD rats were divided into 3 groups randomly: control group （n = 21） ;heart failure group （n = 21） ,NaHS group （n = 21）.The rats in NaHS group were intraperitoneally injected with NaHS（56 μmol/kg） once a day for 12 weeks after heart failure was established,while the other groups were intraperitoneally injected with equal volume of saline.The effect of NaHS on the blood levels of H₂S,MDA,SOD and ox-LDL,and activity of H₂S synthase and contents of MDA,SOD in the myocardium were examined at 4 weeks,8 weeks and 12 weeks after treatment.Results Compared with the heart failure group,the plasma H₂S concentration in NaHS group increased obviously at each occasion.The plasma MDA concentration decreased and plasma SOD concentration increased in the NaHS group,similar results were observed in the myocardium.The plasma ox-LDL concentration decreased in the NaHS group at each occasion.Conclusion The oxidative stress condition can be changed by NaHS intraperitoneal injection in the rat model of pressure-overload heart failure.It may be one of the mechanisms of protecting the heart function by H₂S in heart failure.更多还原