【摘要】 为了进一步解决具有抗癌活性的紫杉醇资源短缺的问题,以红豆杉的幼嫩茎段为外植体筛选出最佳灭菌措施及继代增殖中6-BA、NAA、TDZ不同激素的最佳配比。结论如后:70%(体积百分比,后同)酒精浸泡20 s+0.3%(体积百分数,后同)升汞浸泡6 min灭菌效果最好;最适宜腋芽继代增殖的培养基配方为MS+6-BA 0.15mg/L+NAA1.0 mg/L+TDZ 0.01 mg/L+食糖30 g/L+琼脂8 g/L+AC 0.4 g/L,pH值为5.8。更多还原

【Abstract】 In order to solve the problem of the resources shortage for taxol of anti-cancer activity,based on the experiment of explants from the tender stem segments of Taxus media to choose the optimum technique of sterilization and the best proportion of the subculture with 6-BA,NAA,TDZ different hormones,got results as follows: the optimum technique of explant sterilization of Taxus media was in 70% ethanol for 20 s and 0.3% HgCl2 for 6 min;the most appropriate medium for multiplication axillary bud subculture was MS+6-BA 0.15 mg/L+NAA 1.0 mg/L+TDZ 0.01 mg/L+sugar 30 g/L+Agar 8 g/L+AC 0.4 g/L,pH 5.8.更多还原