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利用RAPD技术快速鉴定辣椒杂交种纯度的研究
Purity Identification of Hot Pepper Hybrids by RAPD
【摘要】 以新椒系列辣椒杂交种父母本和一代杂种为材料,采用液氮-SDS法提取辣椒基因组DNA,研究PCR反应的主要影响因子,建立适合辣椒杂交种纯度RAPD分析的PCR反应体系,从120个随机引物中筛选出1个能鉴定新椒10号杂种纯度的引物S1220,从7个随机引物中筛选出1个能鉴定新椒3号杂种纯度的引物S1213。
【Abstract】 Some F1 hybrids in Xinjiao hot pepper series and their parents were used as experimental materials in this study.The genomic DNA was extracted with liquid N2-SDS method and the PCR system that was suitable for RAPD analysis of these materials was established.The results showed that primer S1220 could test the purity of F1 hybrid A effectively and S1213 could test the purity of F1 hybrid B effectively.
- 【文献出处】 辣椒杂志 ,Journal of China Capsicum , 编辑部邮箱 ,2010年04期
- 【分类号】S641.3
- 【被引频次】4
- 【下载频次】70