【摘要】 考察了4种大孔吸附树脂对仙人掌总黄酮的吸附分离性能,筛选出效果最佳的树脂为AB-8。以总黄酮的吸附量、总黄酮含量和回收率为考察指标,采用紫外分光光度法测定总黄酮。确定了AB-8树脂吸附分离仙人掌总黄酮的工艺条件:上样浓度为15mg/mL,仙人掌总黄酮最大吸附量为18.6mg/mL,吸附流速为5mL/min,洗脱剂为70%乙醇,洗脱剂用量为6倍柱体积,树脂可重复使用3次。经AB-8树脂分离纯化后,总黄酮含量从29%提高到76%,总黄酮回收率为86%。实验结果表明,AB-8树脂可用于仙人掌总黄酮的分离纯化。更多还原

【Abstract】 To assess the capability of 4 kinds of macroporus adsorption resins for absorbing and separating total flavone in cactus.The optimum adsorption results were got by AB-8 resin.The evaluation indexes included total flavone adsorption capacity,total flavone content,and recovery rate.The UV spectrophotometric method was used in determination of total flavone.The technological condition of adsorption and separating total flavone in opuntia dillenii Haw:the extract concentration was 15mg/mL,the maximum adsorption capacity was 18.6mg/mL,the adsorption velocity was 5mL/min,the eluting reagent was 70% ethanol,the dose of the reagent was 6 times as much as the column volume,and the resin could be used three times repeatedly.Total flavone content can be increased from 29% to 76%,with a total flawone recovery rate of 86% following the separation and purification by AB-8 resin.The results indicated that AB-8 resin can be used in separation and purification for total flavone in opuntia dillenii Haw.更多还原