【摘要】 提出了预衍生化高效液相色谱法测定卷烟粘合剂中甲醛、乙醛含量的方法。卷烟粘合剂中醛经纯净水提取,离心机离心后分取部分上层清液,与2,4-二硝基苯肼衍生反应,生成的甲醛和乙醛的2,4-二硝基苯腙,用C18柱进行分离,用乙腈与水的混合物作流动相。在358nm紫外波长下检测,用醛衍生物配制标准定量。甲醛和乙醛在0.10～10.0mg·mL-1范围内呈线性。甲醛加标回收率在98.3%～118.9%之间,乙醛加标回收率在79.2%～89.2%之间,相对标准偏差(n=5)均小于5%。更多还原

【Abstract】 A method for HPLC determination of formaldehyde and acetaldehyde in adhesives for cigarettes,with pre-derivatization using 2,4-dinitrophenylhydrazine as derivatizing agent,was proposed.The adhesive sample was extracted with pure water and the extract was contrifuged.An aliquot of the supernatant was taken and derivatized with the derivatizing agent.The products of derivatization,i.e.,the 2,4-dinitrophenylhydrazone of formaldehyde and acetaldehyde,were separated on C18 column and eluted with mobile phase containing acetonitrile and water.UV detection at 358 nm was adopted in the determination.Standard curves were prepared using standard 2,4-dinitrophenylhydrazones of formaldehyde and acetaldehyde.Linear relationship in the ranges of 0.10-10.0 mg·L-1 were found for both formaldehyde and acetaldehyde.Tests for recovery were made and the results obtained were in the ranges of 98.3%-118.9%(for HCHO)and 79.2%-89.2%(for CH3CHO),with values of RSD’s(n=5)less than 5%.更多还原