【摘要】 建立了叶下珠药材中黄酮的毛细管电泳指纹图谱。以硼砂和十二烷基磺酸钠(pH9.0)为背景电解质溶液,运行电压15kV,紫外检测波长245nm,流体静压力进样15s(高度12cm),对不同产地叶下珠药材进样检测。按电泳峰共有率fi≥70%为依据,确定10个不同产地叶下珠药材中黄酮的毛细管电泳指纹峰为8个,各产地叶下珠的毛细管电泳指纹图谱与标准毛细管电泳指纹图谱的相似度较好。在制备供试液后不同时间进样测定,各指纹峰的相对迁移时间的相对标准偏差小于5%,相对峰面积的相对标准偏差在3.0%～7.8%之间,结果表明样品在48h内稳定。更多还原

【Abstract】 The electrophoretic finger print atlas of flavones in the TCM of phyllanthus urinarial was established by capillary electrophoresis.A mixture of 60 mmol·L-1 borax solution and 16 mmol·L-1 sodium dodecyl sufate solution(adjusted to pH 9 with 0.1 mmol·L-1 boric acid solution preliminarily)was used as background electrolyte,and moving voltage of 15 kV was applied.UV-detection at the wavelength of 245 nm was adopted.Samples were introduced hydrostatically at a height of 12 cm for 15 s.Samples of phyllanthus urinarial grown in 10 different areas were taken for analysis.Base on the value of rate of common electrophoretic peaks fi≥70%,a set of electrophoretic finger print atlas of 8 peaks was determined for flavones in phyllanthus urinarial samples from 10 different areas in our country.Good similarities between the electrophoretic finger print atlases of the samples and the standards were obtained.Relative standard deviation among the values of relative migration times of various finger print peaks obtained at different time of sample introduction was less than 5%,and values of RSD’s for the relative peak areas were in the range from 3.0% to 7.8%.The results obtained proved that the sample was stable within 48 h.更多还原