【摘要】 目的:研究C反应蛋白(CRP)基因的3个单核苷酸多态性(SNP)位点(rs1205r、s1130864、及rs1800947)与原发性高血压病的相关性。方法:采用TaqMan荧光定量PCR技术,对中国上海地区712例高血压病患者和672名正常血压者进行基因分型、分析单位点和单倍型与高血压及表型的相关性。结果:未发现CRP基因的3个SNP位点的基因型和等位基因频率在原发性高血压病组(EH)和正常血压者对照组(NT)之间存在显著差异(P>0.05)。CRP基因rs1205(T/C)基因位点和rs1130864(G/A)基因位点经过连锁不平衡分析,D’值<0.85,进行单倍型分析显示,单倍型C-A分布频率在EH组和NT组中有显著差异[NT比EH:0.064比0.040,优势比(OR)=0.617,95%可信区间(CI):0.429~0.887,P=0.009],并且经过FDR校正后仍具有显著差异。未发现高血压病患者中不同单倍型之间血压、体质量指数和血清超敏CRP存在显著差异。结论:CRP基因3个SNP单点与原发性高血压病无明显相关性,单倍型CA可能与高血压保护作用有关。更多还原

【Abstract】 Objective:To study the relationship between 3 single nucleotide polymorphisms(SNP)of C reactive protein(CRP),rs1205,rs1130864,rs1800947 and essential hypertension.Methods: We performed the genotyping analyses,haplotype analyses and genotype with phenotypic analyses in 712 hypertensive patients(EH group)and 672 normotensive controls(NT group)by using TaqMan fluorescent quantitation PCR methods.Results: No significant difference was observed between single SNPs and hypertension(both P>0.05).The linkage disequilibrium(D′<0.85) was observed between rs1205(T/C) and rs1130864(G/A).The haplotype analysis showed the frequency of C-A haplotype was significantly higher in NT group than in EH group [NT vs.EH: 0.64 vs.0.040;odd ratio(OR)=0.617,95%confidence intervel(CI):0.429-0.887,P=0.009],even after the false discovery rate(FDR) correction.No significance was noted for the association of CA haplotype with serum CRP levels and other phenotypes.Conclusions: There is o significant association between 3 SNPs of CRP gene and essential hypertension.The CA haplotype may contribute to protection for hypertension.更多还原