【摘要】 目的:研究葡萄糖刺激胰岛β细胞后,是否通过活性氧(ROS)调控第10号染色体缺失的磷酸酶与张力蛋白同源物基因(PTEN)表达而参与胰岛素分泌信号通路的调控。方法:用不同浓度葡萄糖和H2O2短时间干预胰岛细胞系(INS-1细胞),分别检测细胞分泌胰岛素、细胞内ROS浓度及PTENmRNA表达。结果:(1)随着刺激的葡萄糖、H2O2浓度升高,INS-1细胞内ROS浓度和分泌的胰岛素逐渐升高。(2)随着葡萄糖刺激加大或H2O2浓度逐渐升高,INS-1细胞内PTENmRNA表达先受到抑制,但当刺激浓度超过一定范围后,抑制作用消失。结论:葡萄糖可能通过刺激胰岛细胞内ROS生成,以低浓度ROS作为信号分子抑制PTEN表达,成为调控胰岛素分泌的机制之一。更多还原

【Abstract】 Objective:To study effect of reactive oxygen species(ROS),induced from hyperglucose,on the expression of PTEN gene in INS-1 cell line.Methods:The cultured INS-1 cell line was exposed to different concentrations of glucose and H2O2 for a short time.Then,concentrations of secreted insulin,ROS and phosphatase and tensin homologue detetell on chromososme ten(PTEN) mRNA levels in cultured cells were detected.Results: Concentrations of ROS in INS-1 cells and secreted insulin increased along with increased glucose or H2O2 exposure.The PTEN mRNA expression decreased along with increased glucose or H2O2 exposure,but when glucose or H2O2 were above a certain concentration,their inhibition on PTEN mRNA expression disappeared.Conclusion: Low ROS derived from glucose metabolism can inhibit the PTEN gene expression,which may be one of insulin secretion mechanisms in INS-1 cells induced by glucose stimulation.更多还原