【摘要】 目的:探讨微柱凝胶技术在新生儿溶血病(HDN)筛查中的应用以及结果分析。方法:采用微柱凝胶技术对633例疑似HDN患儿进行检测,用经典抗球蛋白法做对照。结果:633例筛查实验微柱凝胶技术阳性率为30.5%,28%为ABO-HDN,2.5%为RH-HDN,其中直接抗球蛋白实验阳性率为6.8%,患儿红细胞抗体放散实验阳性率为30.5%(抗A抗体阳性89例,抗B抗体阳性45例,抗AB抗体阳性43例,抗D抗体阳性10例,抗E抗体阳性6例),血清游离抗体鉴定阳性率为13%,而经典抗球蛋白法的直接抗球蛋白实验阳性率为3%,患儿红细胞抗体放散实验阳性率为仅为24.7%。结论:在温州地区的HDN以ABO-HDN最为常见,抗体分布特征为抗A抗体>抗B抗体>抗AB抗体>抗D抗体>抗E抗体。新生儿溶血微柱凝胶技术能快速准确检测出HDN,具有很高的灵敏度,并能够检出经典抗球蛋白法所不能检出的阴性标本,是直观可靠的方法。更多还原

【Abstract】 Objective:To investigate the application of microcolumn gel technique in screening hemolytic disease of the newborn (HDN) and results analysis.Method:633 newborns with suspicious HDN were tested by microcolumn gel technique,compared with traditional tube agglutination technique (TAT) test.Result:Among 633 cases of suspicious HDN,the positive rate of HDN screening test was 30.5%,and 28% were ABO HDN,2.5% were RH HDN,from which the positive rate of direct Coombs’test was 6.8%,elution test was 30.5%( 89 cases of anti-A,45 cases of anti-B,43 cases of anti-AB,10 cases of anti-D,6 cases of anti-E),and serum free antibody rate was 13%,.while the rates of direct Coombs’test and elution test results of traditional TAT anti human IgG test were 3% and 24.7%,respectively.Conclusion:ABO-HDN was common in Wenzhou region,the distribution characteristics of antibody was anti-A>anti-B>anti-AB>anti-D>anti-E.Compared with the traditional TAT test,microcolumn gel technique would be more sensitive,simple and accurate,which could detect the negative case which couldn’t be tested by TAT,so microcolumn gel technique would be a dependable method.更多还原