【摘要】 目的:通过大鼠心肌缺血再灌注损伤(IRI)模型,分析白细胞介素-17(IL-17)在IRI中的作用。方法:用阻断大鼠冠状动脉左前降支的方法制作IRI模型。Realtime-PCR及Western blot方法动态观察IRI后不同时间点心肌组织IL-17的表达水平,流式细胞术进一步检测IL-17的来源。应用抗IL-17抗体体内干预,观察其对IRI的作用。结果:IRI后1h就有IL-17的表达,并且在24h的观察期内持续存在,未有峰值出现。流式细胞术检测结果显示心肌组织中IL-17的主要来源是CD4+T细胞。用抗IL-17的抗体体内干预后,血清肌钙蛋白T的水平降低,心肌梗死面积也明显减小。结论:IL-17参与大鼠IRI过程,中和IL-17能明显减轻心肌IRI。更多还原

【Abstract】 Objective:To investigate the effect of interleukin-17(IL-17) in myocardial ischemia reperfusion injury (IRI) in rat.Method:The myocardial ischemia reperfusion injury model was performed by temporary occlusion of left anterior descending in rats. The expression of IL-17 in myocardial tissue was detected by Real time-PCR and western blot at different times after reperfusion. The source of IL -17 was confirmed by flow cytometry. Rats were treated with anti-IL-17 antibody in vivo for determining if IL -17 was involved in myocardial IRI. Result:The expression of IL-17 was detected as early as 1 hour after reperfusion,lasted for 24 hours,and showed no peak in this period. The results of flow cytometry revealed that CD4+T lymphocyte was a major source of IL-17 in myocardial tissue after reperfusion. Administration of anti-IL-17 resulted in a significant decrease in serum troponin T and myocardial infarct size. Conclusion:IL-17 is involved in the pathogenesis of myocardial IRI.更多还原