【摘要】 目的探讨Survivin(生存素)、突变型p53蛋白和与细胞周期蛋白D1(CyclinD1)在乳腺癌中的表达及三者间的相关性和意义。方法应用免疫组化二步法检测56例乳腺癌及10例乳腺纤维腺瘤Survivin、p53蛋白和CyclinD1表达的阳性率。结果在乳腺癌中Survivin、p53蛋白和CyclinD1的表达阳性率分别为78.6%(44/56)、35.7%(20/56)、71.4%(40/56)。乳腺癌Survivin阳性的44例中40例CyclinD1表达阳性,阳性率为90.9%(40/44);乳腺癌Survivin阴性的12例中CyclinD1表达均阴性,两者有相关性(α=0.6531,P<0.05)。56例乳腺癌中p53蛋白阳性20例,其中15例CyclinD1表达阳性,阳性率75%;p53阴性的36例中25例CyclinD1表达阳性,阳性率为69.4%,两者比较差异无统计学意义(P>0.05)。乳腺纤维腺瘤均不表达Survivin、p53蛋白、CyclinD1。结论Survivin在乳腺癌的发生、发展中起重要作用,可能与乳腺癌细胞分裂增殖密切相关;突变型p53蛋白、CyclinD1与乳腺癌的发生、发展亦有一定的相关性。更多还原

【Abstract】 Objective To study the expression of survivin,p53 protein and cyclinD1 and their relationships in breast carcinoma.Methods Survivin,p53 protein and CyclinD1 were detected by using immunohistochemical two step method.Results The positive expression rates of survivin and p53 and cyclinD1 protein were 78.76% and 35.7% and 71.4% in breast Carcinoma.The positive expression rate of CyclinD1 protein was 90.9% in the 44 breast carcinoma tissues of positive expression of Survivin protein.The positive expression rate of CyclinD1 protein was 0% in the 12 breast carcinoma tissues of negative expression of Survivin protein.The expression of Survivin was positively correlated with expression of CyclinD1(α=0.6531,P<0.05).The positive expression rate of CyclinD1 protein was 75% in the 20 breast carcinoma tissues of positive expression of p53 protein.The positive expression rate of CyclinD1 protein was 69.4% in the 36 breast carcinoma tissues of negative expression of p53 protein.The expression of P53 was negatively correlated with expression of CyclinD1(P>0.05).Survivin,p53 Protein and cyclinD1 was not expressed in fibroadenoma of breast cancer.Conclusion Suvivin protein might play an important role in the oncogenesis and development of beast cancer.Survivin might be closely associated with division and proliferation of breast cancer cells.P53 and CyclinD1 might be associated with the oncogenesis and development of beast cancer.更多还原