【摘要】 目的:探讨咖啡因对慢性低O₂高CO₂处理的大鼠空间学习记忆、皮质和海马N-甲基-D-天冬氨酸受体（NMDAR）亚基1 mRNA（NR1 mRNA）表达的影响。方法:SD大鼠48只分为4组,正常对照组;低O₂高CO₂4周（HH）组,处理组:低O₂高CO₂+咖啡因0.1 mg·mL^-14周（HC1组）,低O₂高CO₂+咖啡因0.3 mg·mL^-14周（HC2组）。处理组以咖啡因水溶液干预4周后行Morris水迷宫实验,观察大鼠寻找站台的平均逃避潜伏期和游泳总距离;采用原位杂交法观察大鼠海马及皮质区NR1 mRNA的表达与分布情况。结果:①Morris水迷宫实验显示,HH组与对照组相比大鼠寻找站台的平均逃避潜伏期延长、游泳总距离增加（P<0.05）,HC2组有显著性降低（P<0.05）;②原位杂交显示NR1 mRNA阳性细胞广泛分布于海马和皮质区;模型组与对照组比较大鼠海马锥体细胞层NR1 mRNA表达的平均吸光度值降低（P<0.05）,而咖啡因处理组平均吸光度值升高。结论:咖啡因可改善慢性低O₂高CO₂大鼠的空间学习记忆能力并增加海马和皮质区NR1 mRNA的表达。更多还原

【Abstract】 Aim:To explore the effects of caffeine on learning-memory and the expression of N-methyl-D-aspartatic receptor subunit 1(NR1 mRNA) in rat model of chronic hypoxic hypercapnia.Methods:Building 48 rats models deal with hypoxic hypercapnia and/or caffein liquor,at the end of 4-week exposure,the mean escape latency and swim path distance of rats were assessed with me Morris water maze.The expressions of NR1 mRNA in the hippocampus and cortex of rats were determined by hybridization in situ.Results:①In the Morris water maze task,the mean escape latency and swim path length of model group were longer than that of the control group (P<0.05),and high dose group interfered with caffeine decrease apparently(P<0.05);②In situ hybridization,the expressions of NR1 mRNA were showed in the hippocampus and the cortex of rats.The optical density(A) of NR1 mRNA in model groups was lower than that of the control group(P<0.05),when interfered with caffeine,it increased gradually.Conclusion:Caffeine could improve spatial learning-memory ability and increase me mRNA expression of NR1 in the hippocampus and the cortex in chronic hypoxic hypercapnia rats model.更多还原