【摘要】 目的:研究激光捕获显微切割及基因扫描定性检测T细胞受体(TCR)γ基因重排在早期蕈样肉芽肿(MF)细胞中表达的可行性。方法:激光捕获显微切割(laser capture microdissection,LCM)分离早期MF中的肿瘤细胞,荧光标记引物双重聚合酶链反应(PCR)和基因扫描(GeneScan)分析TCRγ基因重排。结果:9例早期MF的TCRγ基因重排检出率为66.7%(6/9),2例肿瘤期MF的检出率为100%。结论:激光捕获显微切割技术可用于诊断早期MF的TCRγ基因重排检测。更多还原

【Abstract】 Objective: To investigate the feasibility of laser capture microdissection (LCM) and genescan in T cell receptor gamma gene rearrangement in early mycosis fungoides. Methods: LCM to obtain lymphocytic infiltrates from tissue sections of skin biopsy specimens was used. T cell receptor gamma-chain polymerase chain reaction genescan analysis (TCR-γ-PCR-GSA) was used to ana lyze the clonality status of 9 patients with early MF and 2 patients with tumor-stage MF. Results: A monoclonal T cell infiltrate was demonstrated in 66.7% of early stage MF cases and in 100% of tumor stage MF patients. Conclusion: Applying LCM to clonality analysis of early MF could be more rapid and sensitive than conventional methods, and to introduce this approach into the routine diagnosis is recommended.更多还原