【摘要】 目的:观察碱性成纤维细胞生长因子(bFGF)能否通过血迷路屏障,为临床合理用药提供实验依据。方法:豚鼠30只分为3组:125I-bFGF腹腔注入(125I-bFGF组)10只,125I腹腔注入(125I组)10只,生理盐水腹腔注入(生理盐水组)10只。2h后,取血、脑、双耳蜗、甲状腺、心脏、肝脏、肾脏称重,于r放射免疫分析仪中测定每个样品的每分钟记数率。双耳蜗标本切片做放射自显影检查。结果:125I组各脏器每分钟放射含量最高,耳蜗标本切片放射自显影可见颗粒显现;125I-bFGF组各脏器每分钟放射含量以血中最高,耳蜗及脑内含量最低,耳蜗标本切片放射自显影未见颗粒显现。结论:125I标记的bFGF腹腔注入后透过血迷路屏障有一定困难,因此,bFGF临床用药途径值得探讨。更多还原

【Abstract】 Objective:To observe whether bFGF could cross the blood-labyrinth barrier(BLB)after intra-abdominal injection and to establish an experimental basis for its clinical applications.Method:Thirty guinea pigs were divided into three groups.Animals in group 1were admistered125I-bFGF,while animals in group 2and 3were admistered125I and saline,respectively,via intra-abdominal injection.The both cochlea,blood,liver,brain,thyroid gland and kidney were collected and weighted.A radioimmunoassay analyzer was employed to measure counts per minute(CPM)of each sample,and autoradiography was performed on both cochlea.Result:The CPM value of organ samples in the125I group was higher than that in other groups,and radioactive grain was observed in cochlear samples of this group.In the 125I-bFGF group,blood demonstrated the highest CPM value,while cochlea and brain demonstrated the lowest CPM value,with no radioactive grain observed in cochlear samples.Conclusion:bFGF has some difficulties in getting across BLB,so the way of bFGF application in clinics need further study.更多还原